How to Pour a Polyacrylamide Gel
This scrollable interactive explains how a polyacrylamide gel works and shows the steps involved in pouring a polyacrylamide gel.
Topics
- sodium dodecyl sulfate
- denatures proteins
- positive charge
- negative charge
- serological pipette
- isopropanol layer
- isopropanol
- rubber foam
- acrylamide/bis-acrylamide mix
- double-distilled water
- TEMED
- APS
- acrylamide mix
- Tris
- ammonium persulfate
- tetramethylethylenediamine
- pipette
- storing a gel
- refrigerator
- micropipette
- well
- small protein
- large protein
- protein
- charge
- electrical charge
- electrical
- separation of proteins
- lower molecular weight
- higher molecular weight
- polyacrylamide gel
- small pore
- large pore
- cross-linking
- resolving
- stacking
- SDS
- concentration
- molecular weight
- electrophoresis
- polyacrylamide
- gel
- denature
- bands
- kDa
- protein ladder
- polymerization
- acrylamide
- monomers
- bis-acrylamide
- cross-linker
- pore
- pore size
- stacking gel
- resolving gel
- spacer plate
- short plate
- casing frame
- casting stand
- comb
- wells