Spectrophotometer Virtual Lab: Absorbance and Concentration

In this virtual lab, we show how a spectrophotometer is used to determine the absorbance and concentration of a solution.

Topics

  • spectrophotometer
  • absorbance
  • concentration
  • spectrum
  • light
  • entering light
  • exiting light
  • Beer-Lambert law
  • wavelength
  • solution
  • cuvette
  • blank
  • blank cuvette
  • zeroing
  • sample cuvette
  • dilution
  • diluted sample
  • instrument
  • lab equipment
  • protocol
  • best-fit line
  • standard curve
  • standard
  • linear range
  • molar absorptivity constant
  • scatter plot
  • transmission