How to Interpret a Western Blot: Reading through common issues

This text asset describes common issues that can complicate interpretation of a western blot and describes how to resolve such issues.

Topics

  • western blot
  • western
  • bands
  • molecular weight
  • protein
  • target protein
  • protein of interest
  • cleavage
  • degradation
  • splice variant
  • epitope
  • posttranslational modification
  • protein-protein interaction
  • cross reactivity
  • loading control
  • antibody
  • primary antibody