PCR Primer Design
This interactive will present the rules for designing precise nucleotide sequences called PCR primers. Designing primers for a PCR reaction is an important step because the way they bind to the template DNA dictates much of the success of the PCR reaction.
Topics
- polymerase chain reaction
- pcr
- gene
- amplification
- denature
- denaturation
- pcr product
- gel electrophoresis
- dna sequencing
- dna
- genome
- amplified dna
- pcr cycle
- template
- strand
- forward primer
- reverse primer
- lab technique
- sequence
- specific sequence
- nucleotide
- nucleotide sequence
- region
- design
- primer
- amplify
- anneal
- dna synthesis
- dna polymerase
- enzyme
- reaction
- pcr reaction
- complementary base pairing
- guanines
- cytosines
- hydrogen bond
- taq
- taq polymerase
- elongation
- complementary
- mismatch
- mutation
- base pair
- base pairing
- 5’ to 3’
- 5 prime
- 3 prime
- GC content
- AT content
- melting temperature
- pcr machine
- thermocycler
- annealing
- dissociation
- annealing temperature