Ligating DNA Fragments
This simulation provides the opportunity to practice performing a ligation reaction in a virtual lab setting. This process uses the enzyme DNA ligase to attach two DNA fragments using complementary blunt or sticky ends generated from a restriction enzyme digest.
Topics
- protocol simulation
- Amgen Biotech Experience
- ABE
- ABE Lab 3
- Building a Recombinant Plasmid
- genetic engineering
- biotechnology
- ligation
- DNA
- enzyme
- sticky ends
- restriction enzyme digest
- micropipette
- vortex
- microcentrifuge
- P20
- P-20
- reaction
- molecular biology
- biochemistry