Purification of a Therapeutic Protein
In this simulation, you will design a protein purification experiment that could be used to purify a therapeutic protein. Here, we use a monoclonal antibody as an example of a therapeutic protein.
Topics
- ALXCollab
- A280
- AEX
- affinity chromatography
- anion-exchange chromatography
- antibody structure
- antigen
- bind and elute
- capture
- cation-exchange chromatography
- cell-based impurity
- centrifugation
- CEX
- chromatography
- clarification
- clarified supernatant
- column chromatography
- drug substance process development
- drug substance technology
- drug substance
- eluate
- eluent
- flowthrough
- fraction number
- fraction
- fractionation
- HIC
- host cell
- hydrophobic interaction chromatography
- hydrophobic
- IEX
- impurity
- ion-exchange chromatography
- isoelectric point
- ligand
- mAb structure
- mAb variant
- mAb
- mobile phase
- monoclonal antibody
- pellet
- percent yield
- pI
- polishing 1
- polishing 2
- polishing
- process development
- process-related impurity
- product-related impurity
- protein A affinity chromatography
- protein A
- protein G
- protein gel electrophoresis
- protein of interest
- protein purification
- purification buffer
- purification
- purified mAb
- purified protein
- purity
- resin
- SDS-PAGE
- SDS-PAGE gel
- SEC
- size-based filtration
- size-exclusion chromatography
- stained SDS-PAGE gel
- stationary phase
- supernatant
- therapeutic mAb
- therapeutic monoclonal antibody
- therapeutic protein
- UV chromatogram
- yield