Extracting Plasmid DNA: How To Do a Miniprep

Extracting Plasmid DNA: How To Do a Miniprep

In this method video, Molly takes us into the lab to teach us how to purify plasmid DNA from a liquid culture of bacterial cells. When purifying plasmid DNA from a small volume (3 to 5 ml) of bacterial cells as we do in this video, this technique is called a miniprep. For more information see Building a Recombinant Plasmid: DNA Ligase.

Topics

  • Miniprep
  • technique
  • protocol
  • plasmid
  • E. coli
  • bacteria
  • bacterial prep
  • alkaline lysis
  • extraction
  • plasmid DNA
  • method video