Amplifying Power of PCR
This interactive will present the mathematical consideration of exponentially enriching a piece of DNA using PCR. Typically, a PCR protocol will repeat approximately 30 cycles of denaturation, annealing, and elongation. 30 cycles of PCR produce around 1 billion copies of the amplified region.
Topics
- polymerase chain reaction
- pcr
- gene
- amplification
- denature
- denaturation
- pcr product
- template
- strand
- forward primer
- reverse primer
- lab technique
- sequence
- specific sequence
- nucleotide
- nucleotide sequence
- region
- design
- primer
- amplify
- anneal
- dna synthesis
- dna polymerase
- enzyme
- reaction
- pcr reaction
- complementary base pairing
- guanines
- cytosines
- hydrogen bond
- taq
- taq polymerase
- elongation
- complementary
- base pair
- base pairing
- 5’ to 3’
- 5 prime
- 3 prime
- annealing
- dissociation
- region to amplify
- hydrogen bonding
- oligo
- oligonucleotide
- dntp
- datp
- dctp
- dgtp
- dttp
- deoxyribonucleoside triphosphate
- heat-resistant
- single strand
- annealing temperature
- melting temperature
- template-primer complex
- annealed primer
- 5’-to-3’ direction
- overhang
- exponential
- synthesize
- protocol
- gel electrophoresis
- gel
- dna gel
- dna molecules
- total dna
- desired region
- overhang products