Protein Purification Question Set (Part B)
This question set asks how a nickel column can be used to purify a His-tagged protein, and how SDS PAGE data can be interpreted to determine the success of a protein purification experiment. To learn more about this, see the interactive scrollable about Purifying a Protein.
Topics
- column chromatography
- mixture
- protein purification
- experiment
- flow-through
- gel data
- SDS PAGE
- buffer
- elution
- eluate
- salt wash
- untagged
- affinity chromatography
- chromatography
- bead
- cell disruption pellet
- soluble protein
- supernatant
- pellet
- centrifuge
- lysate
- cleared lysate
- imidazole
- histidine
- His-tag
- ni2+
- column
- nickel column