Purifying Protein by Column Chromatography
This protein purification simulation allows users to explore how a protein of interest, red fluorescent protein, can be purified from cell lysate using hydrophobic column chromatography.
Topics
- protocol simulation
- protein column
- column purification
- column chromatography
- protein chromatography
- protein purification
- stopcock
- bed
- resin
- beads
- separate
- supernatant
- elute
- eluate
- getting what we need
- ABE
- Amgen Biotech Experience
- Lab 6
- buffer
- binding
- elution
- column equilibration
- wash
- lysate
- lysis
- bacteria
- E. coli
- RFP
- red fluorescent protein
- hydrophobic
- protein
- purification
- column
- chromatography