Before Breeding Mice... All About Mutations and Genotypes
Understanding the different kinds of alleles and mutations you might be working with and the genotypes they require is essential before breeding mice. By the end of this lesson, you will be able to define different types of mutations (knock-ins, knock-outs, transgenes) and the genotypes they require. Transcript with screen prints.
Topics
- animal models
- breeding mice
- biomedical research project
- mouse models
- gene
- allele
- mutations
- genotypes
- knock-outs
- knock-ins
- transgenes
- knock-out mutation
- loss-of-function allele
- mRNA
- protein translation
- homozygous
- heterozygous
- knock-in mutation
- targeted insertion
- genome
- point mutation
- loxP sites
- reporting tag
- Gt(ROSA)26Sor locus
- transgene
- DNA sequence
- germ line
- random integration
- hemizygous
- homozygous mice
- literature review
- genetic modifications