How to Interpret a Western Blot: The basics
This text introduces how to interpret western blot to determine the relative size and abundance of a protein of interest, as well as whether a protein of interest interacts with other proteins.
Topics
- western blot
- western
- cell types
- tissue types
- loading control
- antibody
- primary antibody
- protein
- protein expression
- protein level
- protein quantification
- densitometry
- linear range
- oversaturated
- protein-protein interaction
- co-immunoprecipitation
- co-IP
- epitope tags
- Flag