Protein Purification
Specific proteins are either hydrophobic or hydrophilic, depending on the relative amount of hydrophobic and hydrophilic amino acids they contain. This article explains how they can be separated by column chromatography, where a column is packed with small beads that are coated with a material (a resin) that attracts hydrophobic amino acids, and the mixture of proteins is dissolved and passed over the beads. This excerpt is from Chapter 6 of the ABE Student Guide.
Topics
- ABE Lab 6
- Tools and Techniques in Biotechnology
- rfp
- column chromatography
- protein
- red fluorescent protein
- buffer
- DNA
- binding sites
- protein folding
- hydrophobic
- hydrophilic
- amino acids
- separation
- noncovalent
- bonds
- covalent
- elution
- molecular DNA
- ABE
- Amgen Biotech Experience