Using Imidazole to Elute His-tagged Proteins from a Nickel Column
This infographic shows why imidazole can be used to elute a His-tagged protein from a nickel column. To learn more about this, see the interactive scrollable about Purifying a Protein.
Topics
- elution
- elute
- bead
- nickel bead
- nickel ion
- nickel
- protein of interest
- protein purification
- His-tag
- imidazole
- histidine