Bioinformatics Analysis of Quality Scores from FASTQ File Using Biopython

In Day 1 of 12 days of Biopython, I am going to show you what a FASTQ file looks like, and how to read and parse FASTQ file using Biopython. We will then look at the analysis of quality scores and phred quality scores which we will get from the parsed FASTQ file. I am going to show you two graphs to check the distribution of reads and distribution of quality scores. Chapters: 0:00 Intro 0:23 Data we are going to use 0:43 How FASTQ file looks like 1:04 Downloading FASTQ file from 1000 Genomes Project 1:25 Reading and parsing FASTQ file using Biopython SeqIO 1:40 Getting sequence details like identifier, description, sequence, quality scores... 2:05 Phred quality scores 3:15 Checking the distribution of reads per nucleotide 3:38 Distribution of N nucleotide across positions 4:06 Distribution of quality of reads/phred scores 4:35 Box plot of quality scores per position 5:21 Outro You can link to the Github code here. My videos are aimed at everyone interested in Computer Science applications in biology. The mission is to simplify Bioinformatics concepts, one video at a time! Follow me on Twitter.