Restriction Fragment Analysis of the TAS2R38 Gene

In this virtual lab, users get to perform restriction digest and gel electrophoresis to analyze an unknown sample, to determine which alleles of TAS2R38 are present. This lab is designed at an introductory level, and emphasizes the reagents and steps necessary for restriction digest and gel electrophoresis. (Prior knowledge of DNA structure, micropipetting, and PCR is helpful.) To learn more about PCR and explore how a region would have been amplified before analysis, see Using PCR to Amplify a Gene. 

Topics

  • gel electrophoresis
  • restriction enzyme
  • restriction digest
  • TAS2R38
  • SNP
  • single nucleotide polymorphism
  • DNA
  • DNA analysis
  • restriction site
  • PCR
  • amplify
  • agarose gel
  • protocol simulation
  • virtual lab simulation
  • Amgen Biotech Experience
  • precision medicine