Pasting DNA Fragments Together: Ligases

Pasting DNA Fragments Together: Ligases

The role of ligase in gene cloning is similar to its role in replication in that it binds DNA fragments together. In gene cloning, recombinant DNA is the result of ligation of fragments from a restriction digest. This excerpt is from Chapter 3 of the ABE Student Guide.

Topics

  • ligase
  • gene cloning
  • genetic engineering
  • DNA
  • ligation
  • restriction digest
  • recombinant DNA
  • plasmid
  • polymerase
  • ABE
  • Amgen Biotech Experience