How Can We Determine Which DNA Sequences Are Bound by a Protein?
In this simulation, the learner will design a chromatin immunoprecipitation experiment that could be used to determine which DNA sequences are bound by a given protein of interest. The transcription factor, Myc, serves as an example.
Topics
- DNA-binding protein
- E-box
- experimental design
- fold enrichment
- Myc
- target DNA
- target gene
- TF
- transcription factor
- ChIP-PCR
- ChIP-qPCR
- ChIP
- chromatin immunoprecipitation
- chromatin