How to Perform an Immunoblot
This infographic outlines the steps of immunoblotting (Western blotting plus immunodetection), including sample preparation, protein gel electrophoresis, protein transfer, blocking, primary and secondary antibody incubation, and imaging and interpretation. For more detailed explorations, visit the Protein Expression in Gene-edited Cells - Western Blotting pathway on LabXchange, which includes interactives like Western Blot Overview and Western Blot: Testing Protein Expression Levels.
Topics
- immunoblotting
- Western blotting
- Western blot
- immunodetection
- protein gel electrophoresis
- primary antibody
- secondary antibody
- nitrocellulose membrane
- polyvinylidene difluoride membrane
- protein transfer
- protein of interest
- fluorescent probe
- reporter enzyme
- blocking
- blocking reagent
- Western blot imager
- protein bands
- immunoblot interpretation
- protein presence
- protein size
- relative protein amount