Binding Affinity and Kd

The whole premise of biochemistry is that molecules interact to do things. For example(s), the protein enzyme (reaction mediator) DNA Polymerase links together nucleotides (DNA letters) to copy DNA; a protein called tubulin assembles itself into structural supports and molecular conveyor belts in your cells; and proteins called antibodies bind to foreign molecules (like viral proteins) and call for help, etc. Pretty awesome, right? But in order for any of this to happen, the molecules have to first bind one another. Which means they have to: 1) come into contact with one another and ⠀ 2) like each other (first enough to bind and then enough to stay bound) For more information see the Binding Affinity – “Slot/Dot Blot” Filter Binding Assay for Measuring Protein/Nucleic Acid Interactions.