Purifying a Protein
This scrollable interactive introduces how a nickel column can be used to purify a His-tagged protein of interest. The scrollable begins with cloning a His-tag onto a protein of interest. Nickel columns are a commonly used example of affinity chromatography.
Topics
- affinity chromatography
- histidine
- his-tag
- wash buffer
- elution buffer
- lysate
- nickel column
- Ni 2+
- amino acid
- lysis
- sonication
- equilibration buffer
- imidazole
- clear lysate
- flow through
- centrifuge
- expression vector
- protein purification