Polymerase Chain Reaction (PCR)
Polymerase Chain Reaction - or PCR - is known as one of the most ubiquitous and powerful techniques in life science laboratories. It is a highly sensitive and selective method to produce many copies of a particular piece of DNA. This pathway details applications of PCR, its mechanism and primer design, and demonstrates how the method is used in the lab.
Topics
- buffer
- polymerase chain reaction
- pcr
- gene
- amplification
- denature
- denaturation
- pcr product
- template
- strand
- forward primer
- reverse primer
- lab technique
- sequence
- specific sequence
- nucleotide
- nucleotide sequence
- region
- design
- primer
- amplify
- anneal
- dna synthesis
- dna polymerase
- enzyme
- reaction
- pcr reaction
- complementary base pairing
- guanines
- cytosines
- hydrogen bond
- taq
- taq polymerase
- elongation
- complementary
- base pair
- base pairing
- 5’ to 3’
- 5 prime
- 3 prime
- annealing
- dissociation
- magnesium
- ions
- mg2+
- region to amplify
- hydrogen bonding
- oligo
- oligonucleotide
- dntp
- datp
- dctp
- dgtp
- dttp
- deoxyribonucleoside triphosphate
- heat-resistant
- reagent
- potassium
- single strand
- annealing temperature
- melting temperature
- template-primer complex
- annealed primer
- 5’-to-3’ direction
- overhang
- exponential
- synthesize
- protocol
- refrigerate
- gel electrophoresis
- gel
- dna gel
- dna molecules