Protein Purification: Using a Nickel Column to Purify a His-tagged Protein

This pathway describes how a specific type of column chromatography, called affinity chromatography, can be used to purify a His-tagged protein. To learn more about this, see the interactive scrollable about Purifying a Protein.

Topics

pure protein solution
protein of interest
His
His-tag
histidine
enrich
isolate
lysis
ni-nta
nickel ion
chromatography
affinity
protein purification